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	Provedor de dados Braz. J. Vet. Res. Anim. Sci. (1) PAB (1) Autor Reis,Jenner Karlisson Pimenta (2) Andrade,Débora Roque de Freitas (1) Camargos,Marcelo Fernandes (1) Lage,Jéssica (1) Leite,Rômulo Cerqueira (1) Lessa,Leandro Moreira (1) Melo,Marília Martins (1) Nogueira,Márcia Furlan (1) Rezende,Adalgiza Souza Carneiro (1) Rocha,Maurílio Andrade (1) Santiago,Juliano Martins (1) Santos,Sandra Aparecida (1) Stancek,Daniel (1) Trigo,Pablo (1) Mais... Palavra-chave Bovine leukemia virus (1) Brazilian breed (1) Endemic areas (1) Equideoculture (1) Equine infectious anemia (1) Gene (1) Immunodiffusion (1) Physiology of equine exercise (1) Polymerase chain reaction (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/report (1) Ano 2018 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of a polymerase chain reaction and its comparison with agar gel immunodiffusion test in the detection of bovine leukemia virus infection Braz. J. Vet. Res. Anim. Sci. Camargos,Marcelo Fernandes; Stancek,Daniel; Lessa,Leandro Moreira; Reis,Jenner Karlisson Pimenta; Rocha,Maurílio Andrade; Leite,Rômulo Cerqueira. Polymerase chain reaction (PCR) was used for bovine leukemia virus (BLV) detection in the peripheral leukocytes of the infected bovines. The primers used were designed to amplify a part of env gene of BLV. PCR products were analyzed by agarose gel electrophoresis stained by ethidium bromide. The analytical specificity of PCR was confirmed by enzymatic restriction analysis of the PCR product with Bam HI and also by nucleotide sequence analysis of three PCR samples. Sixty five animals were tested for anti-BLV antibody, by agar gel-immunodiffusion test (AGID) and for direct BLV detection by PCR. There was a 73.80% concordance rate between the two tests. Four animals positive in AGID were PCR negative, while 13 AGID negative animals were found PCR positive.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Immunodiffusion; Bovine leukemia virus; Gene; Polymerase chain reaction. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000500005 Equine infectious anemia affects the athletic performance of equines from the Brazilian Pantanal region PAB Andrade,Débora Roque de Freitas; Rezende,Adalgiza Souza Carneiro; Santos,Sandra Aparecida; Nogueira,Márcia Furlan; Santiago,Juliano Martins; Lage,Jéssica; Melo,Marília Martins; Reis,Jenner Karlisson Pimenta; Trigo,Pablo. Abstract: The objective of this work was to evaluate the effects of equine infectious anemia (EIA) on the physical performance of equines from the Brazilian Pantanal region. A total of 16 males were evaluated, divided into two groups: 8 seronegative (G1) and 8 seropositive (G2) for EIA. Two graded exercise tests were carried out before (T1) and after (T2) 42 days of training. Heart rate, lactate concentration, distance covered, and hematocrit level were recorded. In both tests, G1 covered a greater distance. In T2, G2 had lower hematocrit levels and lower speeds reached at different lactate concentrations and heart rates. The athletic performance of the evaluated equines is affected by equine infectious anemia. Tipo: Info:eu-repo/semantics/report Palavras-chave: Brazilian breed; Endemic areas; Equideoculture; Equine infectious anemia; Physiology of equine exercise. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018001001184 Registros recuperados: 2 Primeira ... 1 ... Última

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